毛国斌
毛国斌
  • 职称：
    副研究员
  • 联系方式：
    gb.mao@siat.ac.cn
  • 个人网页：
  • 座右铭：
个人简介

毛国斌,中国科学院深圳先进技术研究材料所副研究员,硕士生导师,深圳市高层次人才。2014年于华中农业大学获应用化学学士学位,2019年于武汉大学获得分析化学博士学位,2019年-2021年在中国科学院深圳先进技术研究院开展博士后研究。近年来主要从事荧光纳米传感与合成生物学传感等相关研究,致力于开发基于量子点等纳米探针与CRISPR/Cas系统等合成生物学系统的超高灵敏生物传感器,实现病毒感染检测、肿瘤早期诊断及慢性疾病监测。以第一(共同第一)/共同通讯作者在J. Am. Chem. Soc., Sci. China-Life Sci., ACS Sens., ACS Appl. Mater. Interfaces, Nano Res., Anal. Chem.等期刊发表论文27篇。在相关领域申请了发明专利13项,授权6项。主持国家自然科学基金青年项目、中国博士后基金面上项目、广东省基础与应用基础区域联合基金等项目4项。作为核心骨干参与科技部国家重点研发计划项目1项。

研究领域

合成生物学传感；纳米生物学传感

代表论著

1. Wen Yin,† Leyao Li,† Yang Yang,† Yuxin Yang, Ruijing Liang, Lixin Ma, Junbiao Dai, Guobin Mao,* and Yingxin Ma* Ultra-Sensitive Detection of the SARS-CoV‐2 Nucleocapsid Protein via a Clustered Regularly Interspaced Short Palindromic Repeat/Cas12a-Mediated Immunoassay, ACS Sensors, 2024, 9, 3150-3157. (SI Cover Story);2. Guobin Mao*, Xing Luo, Silu Ye, Xun Wang, Jin He, Jilie Kong, Junbiao Dai, Wen Yin*, Yingxin Ma*. Fluorescence and Colorimetric Analysis of African Swine Fever Virus Based on the RPA-Assisted CRISPR/Cas12a Strategy. Analytical Chemistry 2023, 95, 8063-8069. (SI Cover Story); 3. Guobin Mao, Silu Ye, Wen Yin, Yang Yang, Xinghu Ji, Jin He, Yingxia Liu, Junbiao Dai, Zhike He*, Yingxin Ma*. Ratiometric Fluorescent Si-FITC Nanoprobe for Immunoassay of SARS-CoV-2 Nucleocapsid Protein. Nano Research 2023, 16, 2859–2865. (Inside Cover Story); 4. Guobin Mao, Guoqiang Wu, Minghai Chen, Chuang Yan, Jingya Tang, Yingxin Ma*, and Xian-En Zhang*. Synthesis of Dual-Emitting CdZnSe/Mn:ZnS Quantum Dots for Sensing the pH Change in Live Cells. Analytical Chemistry 2022, 94, 6665-6671. (SI Cover Story);5. 19. Yingxin Ma†, Guobin Mao†, Weiren Huang†, Guoqiang Wu, Wen Yin, Xinghu Ji, Zishi Deng, Zhiming Cai, Xian-En Zhang, Zhike He*, Zongqiang Cui*. Quantum Dot Nanobeacons for Single RNA Labeling and Imaging. Journal of the American Chemical Society 2019, 141, 13454-13458. (SI Cover Story) 

科研\学术成果

项目:

1.国家自然科学基金青年科学基金项目(22104147),比率型纳米信标的精准构建及HIV-1逆转录过程的动态示踪研究,2022.1-2024.12,30万元,在研,主持。

2.广东省基础与应用基础研究基金(2020A1515111130),多色量子点信标的精准组装及其在多个microRNA同时分析中的应用研究,2020.10-2023.9,10万元,已结题,主持。

3. 中国博士后科学基金面上资助项目(2019M663154),DNA功能化量子点自组装用于乳腺癌精准诊疗的研究,2019.11-2021.7,8万元,已结题,主持。

4. 中国科学院深圳先进技术研究院创新项目-青年人才科研培育项目,双色量子点信标探针的构建及其在HIV逆转录可视化示踪中的应用研究,2021.1-2022.12,10万元,已结题,主持。

5. 科技部国家重点研发计划,基于DNA原理的高密度安全存储系统研发与生物大数据应用示范,2022.1-2024.12,150万,在研,项目骨干。

论文:

1.  Wen Yin,† Leyao Li,† Yang Yang,† Yuxin Yang, Ruijing Liang, Lixin Ma, Junbiao Dai, Guobin Mao,* and Yingxin Ma* Ultra-Sensitive Detection of the SARS-CoV‐2 Nucleocapsid Protein via a Clustered Regularly Interspaced Short Palindromic Repeat/Cas12a-Mediated Immunoassay, ACS Sensors, 2024, 9, 3150-3157. (SI Cover Story)

2.  Guoqiang Wu†, Qiaoyu Li†, Junbiao Dai, Guobin Mao*, Yingxin Ma*. Design and Application of Biosafe Coronavirus Engineering Systems without Virulence. Viruses 2024, 16, 659.

3.  Yao Yu,† Qiaoyu Li,† Wen Shi, Yuxin Yang, Hongpeng He, Junbiao Dai, Guobin Mao,* and Yingxin Ma*, Programmable Aptasensor for Regulating CRISPR/Cas12a Activity, ACS Sensors 2024, 9, 244-250. (SI Cover Story)

4.  Guobin Mao†, Chunmin Qiu†, Xing Luo, Yingqi Liang, Liqing Zhao, Weiren Huang*, Junbiao Dai, Yingxin Ma*. Synergistic effect-triggered fluorescence quenching enables rapid and sensitive detection of alkaline phosphatase, Analytica Chimica Acta 2023, 1272, 341510.

5.  Guobin Mao*, Xing Luo, Silu Ye, Xun Wang, Jin He, Jilie Kong, Junbiao Dai, Wen Yin*, Yingxin Ma*. Fluorescence and Colorimetric Analysis of African Swine Fever Virus Based on the RPA-Assisted CRISPR/Cas12a Strategy. Analytical Chemistry 2023, 95, 8063-8069. (SI Cover Story)

6.  Guobin Mao†, Yang Yang†, Shijie Cao†, Silu Ye, Yifang Li, Wei Zhao, Hongwei An, Yingxia Liu*, Junbiao Dai, and Yingxin Ma*. Ratiometric fluorescence immunoassay of SARS-CoV-2 nucleocapsid protein via Si-FITC nanoprobe-based inner filter effect. Nano Research 2023, 16, 5383–5390. (Back Cover)

7.  Yingqi Liang, Guobing Mao*, Junbiao Dai, Yingxin Ma*. Biofunctionalized semiconductor quantum dots for virus detection. Journal of Semiconductors 2023, 44, 023101.

8.  Guobin Mao, Silu Ye, Wen Yin, Yang Yang, Xinghu Ji, Jin He, Yingxia Liu, Junbiao Dai, Zhike He*, Yingxin Ma*. Ratiometric Fluorescent Si-FITC Nanoprobe for Immunoassay of SARS-CoV-2 Nucleocapsid Protein. Nano Research 2023, 16, 2859–2865. (Inside Cover Story)

9.  Guobin Mao†, Yifang Li†, Guoqiang Wu, Silu Ye, Shijie Cao, Wei Zhao, Junnan Lu, Junbiao Dai, Yingxin Ma*. Construction of ratiometric Si-Mn:ZnSe nanoparticles for the immunoassay of SARS-CoV-2 spike protein. Sensors and Actuators B:Chemical 2022, 369, 132306.

10.  Siqi Huang, Weishan Yang, Silu Ye, Shijie Cao, Yifang Li, Zhaoying Wei, Ka Yan Ngai, Junbiao Dai, Guobin Mao*, Yingxin Ma*. Fluorescence Recovery Based on Synergetic Effect for ALP Detection. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2022, 280, 121550.

11.  Yingxin Ma†*, Guobin Mao†, Weishan Yang†, Guoqiang Wu, Guoqiang Li, Xiaoying Li, Xin Lin, Junnan Lu, Shijun Zhao, Wei Zhao, Junbiao Dai*, Xian-En Zhang*. Studying bona fide SARS-CoV-2 biology in a BSL-2 biosafety environment using a split-virus-genome system. Science China Life Science 2022, 65, 1-4.

12.  Guobin Mao, Guoqiang Wu, Minghai Chen, Chuang Yan, Jingya Tang, Yingxin Ma*, and Xian-En Zhang*. Synthesis of Dual-Emitting CdZnSe/Mn:ZnS Quantum Dots for Sensing the pH Change in Live Cells. Analytical Chemistry 2022, 94, 6665-6671. (SI Cover Story)

13.  Yingxin Ma†*, Guobin Mao†, Guoqiang Wu, and Xian-En Zhang*. Single-Particle Tracking Reveals the Interplay between HIV-1 Reverse Transcription and Uncoating. Analytical Chemistry 2022, 94, 2648-2654. 

14.  Yingxin Ma†, Guobin Mao†, Guoqiang Wu, Minghai Chen, Fujun Qin, Luping Zheng, Xian-En Zhang*.  Dual-Fluorescence Labeling Pseudovirus for Real-Time Imaging of Single SARS-CoV-2 Entry in Respiratory Epithelial Cells. ACS Applied Materials & Interfaces 2021, 13, 24477-24486. (SI Cover Story)

15.  Yingxin Ma†, Guobin Mao†, Guoqiang Wu, Zongqiang Cui*, Xian-En Zhang*, Weiren Huang*. CRISPR-dCas9-Guided and Telomerase-Responsive Nanosystem for Precise Anti-Cancer Drug Delivery. ACS Applied Materials & Interfaces 2021, 13, 7890-7896. (SI Cover Story)

16.  Guobin Mao†, Yingxin Ma†, Guoqiang Wu, Mingyuan Du, Songbai Tian, Siqi Huang, Xinghu Ji, and Zhike He*. Novel Method of Clickable Quantum Dot Construction for Bioorthogonal Labeling. Analytical Chemistry 2021, 93, 777-783.

17. Yingxin Ma†, Guobin Mao†, Guoqiang Wu†, Zhike He, Weiren Huang*. Magnetic bead-enzyme assemble for triple-parameter telomerase detection at single-cell level. Analytical and Bioanalytical Chemistry 2020, 412, 5283-5289.

18.  Yingxin Ma†*, Guobin Mao†, Guoqiang Wu†, Jing Fan, Zhike He, Weiren Huang*. A novel nano-beacon based on DNA functionalized QDs for intracellular telomerase activity monitoring. Sensors and Actuators B: Chemical 2020, 304, 127385.

19. Yingxin Ma†, Guobin Mao†, Weiren Huang†, Guoqiang Wu, Wen Yin, Xinghu Ji, Zishi Deng, Zhiming Cai, Xian-En Zhang, Zhike He*, Zongqiang Cui*. Quantum Dot Nanobeacons for Single RNA Labeling and Imaging. Journal of the American Chemical Society 2019, 141, 13454-13458. (SI Cover Story) 

20.  Yingxin Ma†*, Guobin Mao†, Yucheng Zhong†, Guoqiang Wu, Wanjun Wu, Yonghao Zhan, Zhike He, Weiren Huang*. Highly sensitive ratiometric fluorescent paper sensor for the urine assay of cancer. Talanta 2019, 194, 199-204.  

专利:

1. 马英新,毛国斌,戴俊彪,一种适配体传感器、靶标物的识别方法及应用。CN202311148438.X 申请日:20230906

2. 马英新,毛国斌,戴俊彪,基于ALP介导的ELISA检测IL-6蛋白的方法及试剂盒。CN202310864919.4 申请日:20230713

3. 马英新,毛国斌,戴俊彪,一种荧光硅点的合成方法、荧光硅点及应用。CN202310227436.3 申请日:20230228

4. 马英新,毛国斌,戴俊彪,病毒核酸检测试剂、试剂盒、制备方法及其应用。CN202211618312.X 申请日:20221215

5. 马英新,毛国斌,戴俊彪,用于ELISA检测SARS-CoV-2 S蛋白的比率荧光探针及检测方法。CN202210776581.2

6. 马英新,毛国斌,戴俊彪,用于检测新型冠状病毒的荧光探针及其制备方法与应用。CN202210514610.8

7. 马英新,毛国斌,戴俊彪,Si-FITC比率型荧光纳米探针的合成方法及其在新冠病毒抗原检测中的应用。CN202111564717.5

8. 马英新,毛国斌,戴俊彪,一种镉锌硒量子点及其制备方法和应用、ALP检测方法。CN202111486409.5

9. 戴俊彪,马英新,毛国斌,SARS-CoV-2病毒样颗粒的制备方法及其应用。CN202111271508.1

10. 张先恩,毛国斌,马英新,CdZnSe/Mn:ZnS QDs、合成方法及其应用。ZL202110996152.1